The First Molecular Characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) Vitellogenin Expression Products and a Partial cDNA Sequence Isolation

被引：2

作者：

Costa-Da-Silva, Andre Luis ^{[1
,2
]}

Lorenti, Laisa Sentieri ^{[1
,2
]}

Oliveira, Soraia De Lima ^{[1
,2
]}

Cardoso, Andre Franco ^{[1
]}

Capurro, Margareth Lara ^{[1
,2
]}

机构：

[1] Univ Sao Paulo, Dept Parasitol, BR-05508000 Sao Paulo, Brazil

[2] Univ Fed Rio de Janeiro, INCT EM, BR-21941902 Rio De Janeiro, RJ, Brazil

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2014年 / 51卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

Anopheles aquasalis; vitellogenin; vitellogenesis; mosquito; physiology; GENE-EXPRESSION; MOSQUITO; MALARIA; IDENTIFICATION; ACCUMULATION; PROTEINS; VECTORS;

D O I：

10.1603/ME13045

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial, 886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription profile of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized its translational profile in previtellogenic and vitellogenic females. Our results represent the first molecular characterization of An. aquasalis vitellogenin expression products.

引用

页码：1208 / 1212

页数：5

共 3 条

[1] Isolation and sequence of a partial vitellogenin cDNA from the cockroach, Blattella germanica (L.) (Dictyoptera, Blattellidae), and characterization of the vitellogenin gene expression
Martín, D
Piulachs, MD
Comas, D
Bellés, X
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 1998, 38 (03) : 137 - 146
[2] cDNA cloning, functional expression and characterization of kynurenine 3-hydroxylase of Anopheles stephensi (Diptera: Culicidae)
Hirai, M
Kiuchi, M
Wang, J
Ishii, A
Matsuoka, H
INSECT MOLECULAR BIOLOGY, 2002, 11 (05) : 497 - 504
[3] Molecular characterization of a Chinese soybean mosaic virus isolate by RT-PCR, cDNA sequence analysis and direct expression of PCR products in bacteria
Zhang, JF
Zhao, H
Gui, JG
Liu, KF
Wang, DW
ACTA BOTANICA SINICA, 1999, 41 (09): : 932 - 935

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