Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes

被引：6

作者：

Temperley, Nicholas D. ^{[1
,2
]}

Webster, Lucy M. I. ^{[2
,3
]}

Adam, Aileen ^{[2
]}

Keller, Lukas F. ^{[2
,4
]}

Johnson, Paul C. D. ^{[2
,5
]}

机构：

[1] Roslin Inst Edinburgh, Div Genet & Genom, Roslin EH25 9PS, Midlothian, Scotland

[2] Univ Glasgow, Div Environm & Evolutionary Biol, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[3] Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland

[4] Univ Zurich, Zool Museum, CH-8057 Zurich, Switzerland

[5] Univ Glasgow, Robertson Ctr Biostat, Glasgow G12 8QQ, Lanark, Scotland

来源：

JOURNAL OF PARASITOLOGY | 2009年 / 95卷 / 02期

关键词：

INTERNAL TRANSCRIBED SPACER; PARASITIC NEMATODE; RIBOSOMAL DNA; TELADORSAGIA-CIRCUMCINCTA; RDNA; AMPLIFICATION; STRONGYLIDA; TRIFURCATA; SEQUENCES; DAVTIANI;

D O I：

10.1645/GE-1624.1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.

引用

页码：487 / 489

页数：3

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