Over the past decades, decentralization has become one of the driving forces in the institutional transformation of EU member states. Regions have achieved greater authority both in their own power (self-rule) and in their involvement in decision-making at the national level (shared-rule). Nevertheless, the use of these powers varies greatly across the regions from both a national and a subnational perspective. There is a good reason to suppose that regionalism, which is defined as a movement seeking greater regional autonomy and is primarily expressed by regionalist parties, is one of the main factors of this variability. Testing this hypothesis, we focus on two instruments of shared-rule, the rights of the regions to submit legislative initiatives to the national parliament, and to appeal to the constitutional court against the national authorities. We identified all regionalist parties and their strength in regional legislatures (77 parties in 34 regions), regional initiatives (2610) and appeals (1444) over 2001-2020 in Italy, Germany, Spain, and Switzerland, where all the regions have these rights. OLS regression confirms that the strength of regionalist parties has a positive and statistically significant impact on the activity of the regions in both legislative initiatives and appeals to the court, in combination with the less significant influence of such factors as resource capacity and regional specificities. At the same time, we find substantial cross-national differences in using two instruments of shared-rule.