A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

被引：51
|
作者：
Baud, D. [1 ]
Ladkau, N. [1 ]
Moody, T. S. [2 ]
Ward, J. M. [3 ]
Hailes, H. C. [1 ]
机构：
[1] UCL, Dept Chem, London WC1H 0AJ, England
[2] Dept Biocatalysis & Isotope Chem, Almac, Cratvagon BT63 5QD, North Ireland
[3] UCL, Dept Biochem Engn, London WC1H 0AH, England
来源：
CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 97期
基金：
英国生物技术与生命科学研究理事会;
关键词：
CHIRAL AMINE SYNTHESIS; ASYMMETRIC-SYNTHESIS; OMEGA-TRANSAMINASES; AMINATION; CASCADE;
D O I：
10.1039/c5cc06817g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
引用
收藏
页码：17225 / 17228
页数：4
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