Endophytic Fungi from Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens Possess Inhibitory Activity against Pneumonia Causing Bacteria

被引：2

作者：

Demeni, Paola Cynthia Emoh ^{[1
]}

Betote, Patrick Herve Diboue ^{[1
,2
,3
]}

Kom, Christelle Wayoue ^{[2
]}

Tchamgoue, Eric Ngalani ^{[1
]}

Moni, Esther Del Florence Ndedi ^{[1
]}

Maniepi, Jacqueline Saurelle Foumane ^{[3
]}

Agbor, Gabriel A. ^{[2
]}

Nyegue, Maximilienne Ascension ^{[1
,4
]}

机构：

[1] Univ Yaounde I, Fac Sci, Dept Microbiol, Lab Microbiol, POB 812, Yaounde, Cameroon

[2] Inst Med Res & Med Plants Studies, Ctr Res Med Plants & Tradit Med, Lab Pharmacol, POB 13033, Yaounde, Cameroon

[3] Univ Yaounde I, Fac Med & Biomed Sci, Dept Galen Pharm & Pharmaceut Law, Multidisciplinary Lab, Yaounde, Cameroon

[4] Univ Montpellier, IBMM UMR 5247, CNRS UM, Equipe Glyco & Nanovecteurs Ciblage Therapeut, 15 Ave Charles Flahault,BP 14491, F-34093 Montpellier 5, France

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2021年 / 2021卷

关键词：

ANTIMICROBIAL ACTIVITY; PLANT-EXTRACTS; ANTIOXIDANT; DIVERSITY;

D O I：

10.1155/2021/9966323

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

This study evaluated the antibacterial efficacy of methanolic extracts of isolated endophytic fungi from stem barks and leaves of Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens against Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 35218, responsible for causing pneumonia. The endophytic fungi were isolated and characterized in the Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), and Czapek Dox Agar (CDA) media. The fungi and their methanolic extracts were tested for in vitro antibacterial potential by antagonistic assay for endophytic fungi against bacterial pathogens and microdilution method. The phytochemical screening of extracts was carried out according to the colorimetric and precipitation methods to reveal the presence of secondary metabolites. The results showed that 24 macroscopically and microscopically distinct endophytic fungi were isolated, identified, and stored. These endophytic fungi possessed antibacterial activity against the selected bacterial strains with inhibition zones ranging from 7.00 to 25.00 mm. The endophytic fungi GS(15) and AB(24) have presented the inhibitions zones of 20.33mm and 25.00 mm, respectively, and these were better than the ones obtained for Levofloxacin((R)). The endophytes with inhibition zones greater than 10mm were used for extraction of their secondary metabolites. The endophytic fungi extracts showed antibacterial activity with the minimum inhibitory concentrations (MICs) ranging from 6.25 Chi 10(-4) to 2 Chi 10(-2) g/L and the minimum bactericidal concentrations (MBCs) ranging from 2.5 Chi 10(-3) to 2 Chi 10(-2) g/L. The endophytic fungi GS(15) extract was the most effective extract; it showed bactericidal effects on the tested bacterial strains. The phytochemical screening of the extracts revealed the presence of secondary metabolites classes, responsible for causing the obtained antibacterial activity. Thus, the endophytic fungi methanolic extracts from A. boonei and G. suaveolens have the potential to inhibit the growth of bacteria responsible for nosocomial pneumonia.

引用

页数：10