Site-specific endonuclease from thermophilic Bacillus species MK strain is isoschizomer of SalI

被引:0
|
作者
Kerzhner, MA
Shiryaev, SA
Zheleznaya, LA
Matvienko, NI
机构
[1] RUSSIAN ACAD SCI,INST PROT RES,PUSHCHINO 142292,MOSCOW REGION,RUSSIA
[2] RUSSIAN ACAD SCI,INST THEORET & EXPT BIOPHYS,PUSHCHINO 142292,MOSCOW REGION,RUSSIA
关键词
site-specific endonuclease; isoschizomer; restriction; sequencing;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Screening of thermophilic bacterial strains reveal ed a strain containing site-specific endonuclease BspMK1. This endonuclease was purified to functional homogeneity during sequential chromatographic steps. The enzyme recognizes sequence 5'-G down arrow TCGAC-3' on DNA molecule and is isoschizomer of endonuclease SalI. The molecular mass of BspMKI is about 45 kD. The enzyme is maximally active at 55 degrees C and MRB (50 mM NaCl, 10 mM Tris-HCl, pH 7.4, IO mM MgCl2, 1 mM dithiothreitol) is the optimal buffer. The enzyme is highly stable and retains its activity during two weeks at room temperature.
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收藏
页码:883 / 889
页数:7
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