LC-MS/MS screening strategy for cannabinoids, opiates, amphetamines, cocaine, benzodiazepines and methadone in human serum, urine and post-mortem blood as an effective alternative to immunoassay based methods applied in forensic toxicology for preliminary examination

被引：25

作者：

Dziadosz, Marek ^{[1
]}

Teske, Jorg ^{[1
]}

Henning, Katarina ^{[1
]}

Klintschar, Michael ^{[1
]}

Nordmeier, Frederike ^{[1
]}

机构：

[1] Hannover Med Sch MHH, Inst Legal Med, Carl Neuberg Str 1, D-30625 Hannover, Germany

来源：

FORENSIC CHEMISTRY | 2018年 / 7卷

关键词：

Screening; Immunoassay; LC-MS/MS; Blood; Urine; Serum; TANDEM MASS-SPECTROMETRY; DRUGS; ABUSE; METABOLITES; LIBRARY;

D O I：

10.1016/j.forc.2017.12.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An LC-MS/MS based screening method for cannabinoids, opiates, amphetamines, cocaine, benzodiazepines and methadone was developed for the analysis of human serum, urine and post-mortem blood and discussed as an effective alternative to immunoassay based screening methods. Two mass spectrometric systems with differences in sensitivity common known were used for method validation. A simple protein precipitation was applied for a sample volume of 100 lL analysed using a Luna 5 mm C18 (2) 100 A, 150 mm x 2 mm analytical column and two mobile phases consisting of A (H2O/methanol = 95/5, v/v) and B (H2O/methanol = 3/97, v/v), both with 10 mM ammonium acetate and 0.1% acetic acid. The results achieved demonstrated that the method presented fulfils the recommendations for qualitative screening methods and can be applied successfully in the analysis of real samples. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：33 / 37

页数：5