Distribution of chondroitin sulfate in human endometrium

被引：18
|
作者：
Nasciutti, Luiz E.
Ferrari, Renato
Berardo, Plinio T.
Souza, Maisa L. S.
Takiya, Christina M.
Borojevic, Radovan
Abrao, Mauricio S.
Silva, Luiz-Claudio F.
机构：
[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Histol & Embriol, BR-21941590 Rio De Janeiro, RJ, Brazil
[2] Univ Fed Rio de Janeiro, Hosp Clementino Fraga Filho, Lab Tecido Conjuntivo, Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Inst Bioquim Med, Rio De Janeiro, Brazil
[4] Univ Sao Paulo, Dept Obstet & Ginecol, Sao Paulo, Brazil
来源：
MICRON | 2006年 / 37卷 / 06期
关键词：
chondroitin sulfate; glycosaminoglycan; human endometrium;
D O I：
10.1016/j.micron.2005.12.005
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Sulfated glycosaminoglycan (GAG) composition was characterized in the human endometrium during proliferative and secretory phases of the menstrual cycle. Sulfated GAGs were analyzed in endometrium tissue using metachromatic staining, biochemical analysis including electrophoresis before and after specific enzymatic or chemical degradations, and immunostaining with an antibody against chondroitin sulfate (CS). Our results showed that CS was the main sulfated GAG species detected, accompanied by small amounts of heparan sulfate and dermatan sulfate. CS was distributed overall the connective stroma, around arteriole vessels and glands, and there was no important difference in the immunostaining between the proliferative and secretory endometrium phases. Our findings extend previous observations on the GAG composition in the human endometrium providing new information regarding the tissue distribution and location of endometrial CS. (c) 2005 Elsevier Ltd. All rights reserved.
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页码：544 / 550
页数：7
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