Comparison of the differential regulation of T and B-lymphocyte subsets in the skin and lymph nodes amongst three cattle breeds as potential mediators of immune-resistance to Rhipicephalus microplus

被引：12

作者：

Robbertse, Luise ^{[1
]}

Richards, Sabine Annette ^{[1
]}

Clift, Sarah Jane ^{[2
]}

Barnard, Annette-Christi ^{[1
]}

Leisewitz, Andrew ^{[3
]}

Crafford, Jan Ernst ^{[4
]}

Maritz-Olivier, Christine ^{[1
]}

机构：

[1] Univ Pretoria, Fac Nat & Agr Sci, Dept Genet, Pretoria, South Africa

[2] Univ Pretoria, Dept Paraclin Sci, Fac Vet Sci, Pretoria, South Africa

[3] Univ Pretoria, Dept Compan Anim Clin Studies, Fac Vet Sci, Pretoria, South Africa

[4] Univ Pretoria, Dept Vet Trop Dis, Fac Vet Sci, Pretoria, South Africa

来源：

TICKS AND TICK-BORNE DISEASES | 2018年 / 9卷 / 04期

关键词：

Rhipicephalus microplus; Tick resistance; Lymph node; Skin; Cattle; Immune response; TICK BOOPHILUS-MICROPLUS; ANTIGEN-PRESENTING CELLS; BOS-TAURUS; MAST-CELLS; HYPERSENSITIVITY TEST; REPEATED INFESTATIONS; CELLULAR-RESPONSES; CYTOKINE RESPONSES; IXODES-SCAPULARIS; GENE-EXPRESSION;

D O I：

10.1016/j.ttbdis.2018.03.034

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Although varying natural resistance to ticks between highly resistant Brahman (Bos taurus indicus), resistant Bonsmara (5/8 B. t. indicus x 3/8 B. t. taurus) and susceptible Holstein-Friesian (B. t. taurus) breeds is documented in skin and blood, little information is available describing draining lymph nodes. To elucidate the cellular dynamics during Rhipicephalus microplus induced immune responses, this study analysed immune factors from these cattle breeds using histology, immunohistochemistry and flow cytometry. Following the collection of skin and lymph node samples before artificial tick infestation, cattle were infested with R. microplus larvae. Subsequent sampling coincided with the tick larvae and adult developmental stages. A significant influx of CD20+ B-lymphocytes in the dermis all cattle breeds was observed while CD3+ T-lymphocytes were significantly increased for more tick resistant breeds. Eosinophil infiltration in germinal centres of lymph nodes was significant for all cattle breeds while tingible body macrophages were significantly increased for adult infested Brahman animals. A negligible fluctuation in CD20+ and CD79 alpha+ B-lymphocyte numbers was present in the lymph node of more resistant cattle breeds, while susceptible animals showed a decrease in B-lymphocytes after infestation, followed by an increase between larvae to adult infested time points. Increased variability of gamma d Tlymphocyte populations in lymph nodes was correlated with tick susceptibility. In addition, a more stable T helper lymphocyte population was identified in the lymph nodes for the Brahman cattle breed. Results suggest the association of tick susceptibility with differential B-lymphocyte regulation in lymph node tissues, increased variability of WC1 + gamma delta T-lymphocyte populations in the lymph node as well as a decrease in T helper lymphocytes in the lymph node.

引用

页码：976 / 987

页数：12