Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography

被引：5

作者：

Agbaba, D ^{[1
]}

Mihajlovic, A ^{[1
]}

Beljanski, P ^{[1
]}

ZivanovStakic, D ^{[1
]}

Vladimirov, S ^{[1
]}

机构：

[1] TORLAK INST IMMUNOL & VIROL, YU-11221 BELGRADE, YUGOSLAVIA

来源：

CHROMATOGRAPHIA | 1997年 / 45卷 / 1期

关键词：

thin-layer chromatography; column liquid chromatography; PPD-tuberculin solution; 8-hydroxyquinoline sulfate-preservative;

D O I：

10.1007/BF02505552

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

TLC and HPLC methods for the determination of the preservative, 8-hydroxyquinoline sulfate in PPD-T tuberculin solution were developed. The planar chromatography method involved separation of 8-hydroxyquinoline sulfate on a TLC plate using a butyl-acetate: formic acid :2-propanol mobile phase, detection and quantitation by densitometric scanning. The HPLC method was on a LiChrosorb RP-18 column with acetonitrile-water (65:35 v/v) mobile phase, adjusted to pH 3.05 by phosphoric acid. Linearity, reproducibility and accuracy were found to be satisfactory. Under selected conditions, the limit of detection (LOD) of both methods was similar - about 25 ng.

引用

页码：145 / 148

页数：4

共 50 条

[1] Determination of 8-hydroxyquinoline sulfate in tuberculin solutions by planar and high performance liquid chromatography
D. Agbaba
A. Mihajlovic
P. Beljanski
D. Zivanov-Stakic
S. Vladimirov
[J]. Chromatographia, 1997, 45
[2] INTERACTION OF 8-HYDROXYQUINOLINE SULFATE WITH COMPONENTS OF TUBERCULIN PURIFIED PROTEIN DERIVATIVE SOLUTIONS III - BINDING OF 8-HYDROXYQUINOLINE BY TUBERCULIN PURIFIED PROTEIN DERIVATIVE
HELD, HR
LANDI, S
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1974, 63 (08) : 1205 - 1211
[3] 8-HYDROXYQUINOLINE SULFATE AS A PRESERVATIVE FOR TUBERCULIN PPD (MANTOUX)
PIVNICK, H
SIEBENMANN, CO
LANDI, S
ASHFORD, WR
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1965, 54 (04) : 640 - +
[4] DETERMINATION OF COBALT, CHROMIUM AND VANADIUM AS 8-HYDROXYQUINOLINE COMPLEXES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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[J]. ANALYST, 1984, 109 (06) : 699 - 701
[5] INTERACTION OF 8-HYDROXYQUINOLINE SULFATE WITH COMPONENTS PRESENT IN A TUBERCULIN PPD SOLUTION .1. BINDING OF 8-HYDROXYQUINOLINE BY POLYSORBATE 80
LANDI, S
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[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1966, 55 (01) : 18 - &
[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF 8-HYDROXYQUINOLINE CHELATES OF ALUMINUM AND COBALT(III)
HAMBALI, CS
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[J]. CHROMATOGRAPHIA, 1980, 13 (10) : 633 - 634
[7] INTERACTION OF 8-HYDROXYQUINOLINE SULFATE WITH COMPONENTS PRESENT IN A TUBERCULIN PPD SOLUTION 2
LANDI, S
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[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1968, 57 (04) : 605 - &
[8] REPORT ON 8-HYDROXYQUINOLINE SULFATE
GRANT, EH
ALLISON, AM
[J]. JOURNAL OF THE ASSOCIATION OF OFFICIAL AGRICULTURAL CHEMISTS, 1945, 28 (04): : 699 - 704
[9] REPORT ON 8-HYDROXYQUINOLINE SULFATE
GRANT, EH
[J]. JOURNAL OF THE ASSOCIATION OF OFFICIAL AGRICULTURAL CHEMISTS, 1946, 29 (03): : 280 - 284
[10] High-performance liquid chromatographic assay of 8-hydroxyquinoline sulfate and its stability in immunobiological preparations
Mihajlovic, A
Agbaba, D
Zivanov-Stakic, D
Ristic, P
Dordjevic, M
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 798 (1-2) : 173 - 177

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