An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines

被引：21
|
作者：
He, Yong [1 ,2 ]
Yu, Junli [3 ]
Hu, Xiangzi [1 ,2 ]
Huang, Shumei [1 ,2 ]
Cai, Lili [1 ,2 ]
Yang, Liu [4 ,5 ,6 ]
Zhang, Huatang [1 ,2 ]
Jiang, Yin [1 ,2 ]
Jia, Yongguang [7 ]
Sun, Hongyan [4 ,5 ,6 ]
机构：
[1] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangdong Univ Technol, Sch Biomed & Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Ultrasonog, Guangzhou 510655, Peoples R China
[4] City Univ Hong Kong, Dept Chem, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China
[5] City Univ Hong Kong, Ctr Super Diamond & Adv Films COSDAF, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China
[6] City Univ Hong Kong, Chengdu Res Inst, Chengdu 610200, Peoples R China
[7] South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 87期
基金：
中国国家自然科学基金;
关键词：
Herein; a new fluorescent probe; AE-Phos; is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity; a large Stokes shift; and a fast response towards ALP. Furthermore; AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively. This journal is © The Royal Society of Chemistry;
D O I：
10.1039/d0cc06129h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Herein, a new fluorescent probe, AE-Phos, is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity, a large Stokes shift, and a fast response towards ALP. Furthermore, AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively.
引用
收藏
页码：13323 / 13326
页数：4
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