Preparation and evaluation of partially-substituted 3-chloro-4-methylphenylcarbamate-β-cyclodextrin bonded silica particles as chiral stationary phase for multi-mode HPLC

3-Chloro-4-methylphenylcarbamate-(3-(2-O-beta-cyclodextrin)-2-hydroxypropoxy)-propylsilyl-appended silica particles (CMP-CD-HPS), a new type of partially-substituted cyclodextrin-bonded phase, have been successfully synthesized and applied as a chiral stationary phase in high-performance liquid chromatography under multi-mode conditions including normal phase, reversed phase, and polar organic mobile phase conditions. Chemical characterization for the CMP-CD-HPS was carried out by elemental analysis. The chromatographic performance of CMP-CD-HPS-packed column was evaluated by separating the positional isomers of nitrophenol and nitraniline and the enantiomers of some chiral drug compounds. The separation results show that CMP-CD-HPS exhibited excellent selectivity for separating the positional isomers of disubstituted benzenes and the enantiomers of the chiral drugs. The hydroxyl residues of partially-substituted beta-cyclodextrin and the stable spacer linking to secondary hydroxyl site of the beta-cyclodextrin in the new CMP-CD-HPS phase play important roles in the chromatographic separations.