Identification of bacteriophage types and their carriage in Staphylococcus aureus

被引:73
|
作者
Pantucek, R [1 ]
Doskar, J [1 ]
Ruzicková, V [1 ]
Kaspárek, P [1 ]
Orácová, E [1 ]
Kvardová, V [1 ]
Rosypal, S [1 ]
机构
[1] Masaryk Univ, Fac Sci, Dept Genet & Mol Biol, CS-61137 Brno, Czech Republic
关键词
D O I
10.1007/s00705-004-0335-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Conserved genomic sequences distinctive of Staphylococcus aureus phage types 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages, but also to detect prophages in S. aureus genomes. One to four different prophages were unambiguously detected in experimentally lysogenized S. aureus strains, and substantial variation in prophage content was found in 176 S. aureus clinical strains of different provenance. In addition, by using a comparative genomics approach, all the prophages in the S. aureus genomes sequenced to date could be revealed and classified.
引用
收藏
页码:1689 / 1703
页数:15
相关论文
共 50 条
  • [1] Identification of bacteriophage types and their carriage in Staphylococcus aureus
    R. Pantůček
    J. Doškař
    V. Růžičková
    P. Kašpárek
    E. Oráčová
    V. Kvardová
    S. Rosypal
    Archives of Virology, 2004, 149 : 1689 - 1703
  • [2] Reclassification of Staphylococcus aureus Nasal Carriage Types
    van Belkum, Alex
    Verkaik, Nelianne J.
    de Vogel, Corne P.
    Boelens, Helene A.
    Verveer, Jeroen
    Nouwen, Jan L.
    Verbrugh, Henri A.
    Wertheim, Heiman F. L.
    JOURNAL OF INFECTIOUS DISEASES, 2009, 199 (12): : 1820 - 1826
  • [3] SKIN AND NOSE CARRIAGE OF BACTERIOPHAGE TYPES OF STAPH-AUREUS
    WILLIAMS, REO
    JOURNAL OF PATHOLOGY AND BACTERIOLOGY, 1946, 58 (02): : 259 - 268
  • [4] Bacteriophage-based identification of Staphylococcus aureus
    Idelevich, E. A.
    Walther, T.
    Molinaro, S.
    Wieser, A.
    Peters, G.
    Becker, K.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 303 : 8 - 8
  • [5] BACTERIOPHAGE TYPES AND ANTIBIOTIC SUSCEPTIBILITY OF STAPHYLOCOCCUS-AUREUS
    KLASTERSKY, J
    BEUMER, J
    DANEAU, D
    APPLIED MICROBIOLOGY, 1971, 22 (06) : 1000 - +
  • [6] Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated
    McCarthy, Alex J.
    Witney, Adam A.
    Lindsay, Jodi. A.
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2012, 2
  • [7] ISOLATION AND IDENTIFICATION OF NEW STAPHYLOCOCCUS-AUREUS BACTERIOPHAGE
    DAZA, RM
    PEREA, EJ
    ANNALES DE MICROBIOLOGIE, 1974, B125 (01): : 119 - 122
  • [8] CARRIAGE OF STAPHYLOCOCCUS AUREUS IN PSORIASIS
    NOBLE, WC
    SAVIN, JA
    BRITISH MEDICAL JOURNAL, 1968, 1 (5589): : 417 - &
  • [9] Decolonization of Staphylococcus aureus carriage
    Botelho-Nevers, E.
    Gagnaire, J.
    Verhoeven, P. O.
    Cazorla, C.
    Grattard, F.
    Pozzetto, B.
    Berthelot, P.
    Lucht, F.
    MEDECINE ET MALADIES INFECTIEUSES, 2017, 47 (05): : 305 - 310
  • [10] Carriage of Staphylococcus aureus in the elderly
    Parnaby, RM
    ODwyer, G
    Monsey, HA
    Shafi, MS
    JOURNAL OF HOSPITAL INFECTION, 1996, 33 (03) : 201 - 206