Preparation of hydrophilic size exclusion packings using monodisperse poly (glycidylmethacrylate-co-ethylenedimethacrylate) beads as matrix and their application for biopolymers separation

Based on monodisperse poly ( glycidylmethacrylate-co-ethylenedimethacrylate) particles as a media, a new hydrophilic stationary phase for size exlusion chromatography (SEC) was synthesized by a new chemically modified method. The content of hydroxyl was measured to be 6.1 mmol/g. The modified resin was evaluated for mass recovery of proteins, hydrophilicity, rigidity, and chemical stability in detail. With trihydroxymethylaminomethane ( Tris) buffer ( pH = 7) as mobile phase, three kinds of proteins and a peptide were selected to test the synthesized SEC column. It was abserved that it follows SEC mechanism and a satisfactory result was obtained.