Characterization and genome analysis of the Bacillus cereus-infecting bacteriophages BPS10C and BPS13

被引:8
|
作者
Shin, Hakdong [1 ,2 ]
Lee, Ju-Hoon [3 ]
Park, Jaeeun [1 ,2 ]
Heu, Sunggi [4 ]
Ryu, Sangryeol [1 ,2 ]
机构
[1] Seoul Natl Univ, Res Inst Agr & Life Sci, Ctr Food & Bioconvergence, Dept Food & Anim Biotechnol, Seoul 151921, South Korea
[2] Seoul Natl Univ, Dept Agr Biotechnol, Ctr Food & Bioconvergence, Res Inst Agr & Life Sci, Seoul 151921, South Korea
[3] Kyung Hee Univ, Grad Sch Biotechnol, Dept Food Sci & Biotechnol, Yongin 446701, South Korea
[4] Rural Dev Adm, Natl Acad Agr Sci, Microbial Safety Div, Suwon 441707, South Korea
基金
新加坡国家研究基金会;
关键词
SEQUENCE; THERAPY; LYSINS; PHAGE;
D O I
10.1007/s00705-014-2030-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Due to the emergence of antibiotic-resistant strains, bacteriophages are considered to be an alternative approach for the control of pathogens. In this study, the bacteriophages BPS10C and BPS13 were isolated and characterized to investigate their ability to control food-borne pathogenic Bacillus cereus. Phage BPS13 exhibited slightly higher host lysis activity compared with phage BPS10C. In addition, phage BPS13 exhibited greater stability under various pH and temperature conditions. To extend our knowledge of the lysis of B. cereus by these phages, their genomes were completely sequenced and analyzed, revealing that these phage genomes encode endolysin and two tail lysins, which are likely involved in host lysis and invasion mechanisms, respectively. These lysis-related proteins may increase the bactericidal activities of these phages, suggesting that they may be good candidates for the potential control of B. cereus.
引用
收藏
页码:2171 / 2175
页数:5
相关论文
共 3 条
  • [1] Characterization and genome analysis of the Bacillus cereus-infecting bacteriophages BPS10C and BPS13
    Hakdong Shin
    Ju-Hoon Lee
    Jaeeun Park
    Sunggi Heu
    Sangryeol Ryu
    [J]. Archives of Virology, 2014, 159 : 2171 - 2175
  • [2] Characterization and comparative genomic analysis of bacteriophages infecting members of the Bacillus cereus group
    Ju-Hoon Lee
    Hakdong Shin
    Sangryeol Ryu
    [J]. Archives of Virology, 2014, 159 : 871 - 884
  • [3] Characterization and comparative genomic analysis of bacteriophages infecting members of the Bacillus cereus group
    Lee, Ju-Hoon
    Shin, Hakdong
    Ryu, Sangryeol
    [J]. ARCHIVES OF VIROLOGY, 2014, 159 (05) : 871 - 884