Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation

被引：4

作者：

Monroy-Morales, Elizabeth ^{[1
]}

Davila-Delgado, Raul ^{[1
]}

Ayala-Guzman, Emmanuel ^{[1
]}

Gamboa-deBuen, Alicia ^{[2
]}

Sanchez-Lopez, Rosana ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Ecol, Ciudad De Mexico 04510, Mexico

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 09期

关键词：

cell division; cell plate; cytokinesis; infection thread; nodulation; nucleus; Phaseolus vulgaris; rhizobia; KNOLLE; MEDICAGO-TRUNCATULA; LOTUS-JAPONICUS; TRANSCRIPTION FACTOR; NODULE DEVELOPMENT; AUXIN TRANSPORT; ROOT HAIRS; RHIZOBIAL INFECTION; SMALL GTPASE; TIP GROWTH; PLANT;

D O I：

10.3390/ijms23095267

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes place by inducing cortical cell division (CCD) at the infection site. Genetic analysis showed that both events are well-coordinated; however, the dynamics connecting them remain to be elucidated. To visualize the crossroads between IT and CCD, we benefited from the fact that, in Phaseolus vulgaris nodulation, where the first division occurs in subepidermal cortical cells located underneath the infection site, we traced a Rhizobium etli strain expressing DsRed, the plant cytokinesis marker YFP-PvKNOLLE, a nuclear stain and cell wall auto-fluorescence. We found that the IT exits the root hair to penetrate an underlying subepidermal cortical (S-E) cell when it is concluding cytokinesis.

引用

页数：19