A modified live Mycoplasma gallisepticum vaccine to protect chickens from respiratory disease

被引:24
|
作者
Papazisi, L
Silbart, LK
Frasca, S
Rood, D
Liao, X
Gladd, M
Javed, MA
Geary, SJ [1 ]
机构
[1] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06268 USA
[2] Univ Connecticut, Ctr Excellence Vaccine Res, Storrs, CT USA
[3] Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA
关键词
Mycoplasma gallisepticum; respiratory disease; vaccine;
D O I
10.1016/S0264-410X(02)00372-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of this study was to assess the efficacy of a modified live Mycoplasma gallisepticum vaccine (GT5) for the protection of chickens against infection and respiratory disease. GT5 was constructed by the reconstitution of the avirulent high passage R (R-high) strain with the gene encoding the major cytadhesin GapA. GT5 expressed GapA on its surface yet retained the phenotypic characteristics of the parental R-high strain. Birds vaccinated with GT5 were protected upon challenge with the virulent low passage R (R-low) strain as evidenced by a complete absence of tracheal lesions 2 and 4 weeks post-challenge, in contrast to sham immunized/challenged control birds. Modest amounts of IgG, and little, if any secretory IgA or IgM anti-M. gallisepticum were found in tracheal washings following vaccination. However, copious amounts of specific IgA were found following challenge, especially in sham immunized birds. This suggests that the tracheal IgG elicited by GT5 vaccination may have been responsible for blocking the initial colonization of R-low, thereby resulting in protection. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:3709 / 3719
页数:11
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