New methods for proteomic research: Preparation of proteins with N-terminal cysteines for labeling and conjugation

被引：0
|
作者：
Tolbert, TJ
Wong, CH
机构：
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2002年 / 41卷 / 12期
关键词：
amino acids; chemoselectivity; nanotechnology; proteins; proteomics;
D O I：
10.1002/1521-3773(20020617)41:12<2171::AID-ANIE2171>3.0.CO;2-Q
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The highly selective tobacco etch virus NIa protease (TEV protease) can be used to remove affinity tags with mutated cleavage sites to produce proteins for site-specific labeling and chemoselective ligations (see scheme). This provides a simple, efficient method for producing proteins with N-terminal cysteines for proteomic applications.
引用
收藏
页码：2171 / 2174
页数：4
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