RAPD-based genetic relationships in different Bougainvillea cultivars

被引:11
|
作者
Srivastava, Richa [2 ]
Shukla, Sudhir [1 ]
Soni, Arvind [2 ]
Kumar, Anil [2 ]
机构
[1] Natl Bot Res Inst, Genet & Plant Breeding Sect, Lucknow 226001, Uttar Pradesh, India
[2] Natl Bot Res Inst, Plant Genom Lab, Lucknow 226001, Uttar Pradesh, India
来源
CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2009年 / 9卷 / 02期
关键词
Bougainvillea; RAPD; hybridity; genetic diversity; Principle Component Analysis; IDENTIFICATION; CYTOGENETICS; ROSE;
D O I
10.12702/1984-7033.v09n02a08
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The present study deals with authenticating existing knowledge about 21 Bougainvillea cultivars comprising of 9 hybrids and their parents through RAPD analysis. The 19 degenerate primer sets generated 234 bands from which 158 (67.5%) were polymorphic. The UPGMA based dendrogram divided 21 cultivars into two major groups with Jaccards similarity coefficient ranging from 0.51 to 0.942. Group A had three cultivars namely Trinidad, Formosa and Dr. H. B. Singh in which Dr. H.B. Singh was confirmed as a hybrid of other two cultivars. Group B was sub divided into 8 clusters. The parentages of 7 out of 8 hybirds have been confirmed based on clusters. The study concluded that the RAPD technique is suitable for confirmation of parent-hybrid relationship.
引用
收藏
页码:154 / 163
页数:10
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