Immunoquantitation of cytochromes P450 1A and P450 2B and comparison with chlorinated hydrocarbon levels in archived polar bear liver samples

被引：25
|
作者：
Bandiera, SM [1 ]
Torok, SM [1 ]
Letcher, RJ [1 ]
Norstrom, RJ [1 ]
机构：
[1] CANADIAN WILDLIFE SERV, NATL WILDLIFE RES CTR, HULL, PQ K1A 0H3, CANADA
来源：
CHEMOSPHERE | 1997年 / 34卷 / 5-7期
关键词：
D O I：
10.1016/S0045-6535(97)00443-8
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The present study examined the utility of an immunoblot method for quantitation of cytochrome P450 isozymes in archived liver samples as a bioassay of exposure to halogenated hydrocarbons. Hepatic microsomes were prepared from 44 archived polar bear (Ursus maritimus) liver homogenates that had been stored at approximately -40 degrees C for 9-10 years and analyzed on blots probed with antibodies to rat cytochromes P450 1A1 and P450 2B1. The results revealed a positive correlation between cytochrome P450 1A and total polychlorinated biphenyl (PCB) levels in the archived liver samples, suggesting that cytochrome P450 1A was induced in polar bears by environmental exposure to PCBs. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：1469 / 1479
页数：11
相关论文
共 50 条
  • [1] Upregulation of cytochromes P450 2B in rat liver by orphenadrine
    Murray, M
    Fiala-Beer, E
    Sutton, D
    BRITISH JOURNAL OF PHARMACOLOGY, 2003, 139 (04) : 787 - 796
  • [2] Induction of cytochromes P450 1A, 2B and 2E in hamster tissues by acetone
    Chen, RM
    Ueng, TH
    ARCHIVES OF TOXICOLOGY, 1997, 71 (08) : 489 - 495
  • [3] Induction of cytochromes P450 1A, 2B and 2E in hamster tissues by acetone
    Ruei-Ming Chen
    Tzuu-Huei Ueng
    Archives of Toxicology, 1997, 71 : 489 - 495
  • [4] Functional Characterization of Cytochromes P450 2B from Woodrats
    Wilderman, P. Ross
    Jang, Hyun-Hee
    Angermeier, Elizabeth
    Malenke, Jael R.
    Dearing, M. Denise
    Halpert, James R.
    FASEB JOURNAL, 2012, 26
  • [5] Molecular modeling of cytochromes P450 2B and 3A.
    Szklarz, GD
    FASEB JOURNAL, 1997, 11 (09): : A778 - A778
  • [6] CATALYTIC AND IMMUNOLOGICAL CHARACTERIZATION OF HEPATIC AND LUNG CYTOCHROMES P450 IN THE POLAR BEAR
    BANDIERA, SM
    TOROK, SM
    LIN, S
    RAMSAY, MA
    NORSTROM, RJ
    BIOCHEMICAL PHARMACOLOGY, 1995, 49 (08) : 1135 - 1146
  • [7] Rate distributions in CO geminate rebinding with cytochromes P450(CAM), P450(LM2) and P450(SCC).
    Tetreau, C
    Lavalette, D
    diPrimo, C
    Hoa, GHB
    Lange, R
    BIOPHYSICAL JOURNAL, 1996, 70 (02) : MPMG6 - MPMG6
  • [8] Catalytic modulation of human cytochromes P450 17A1 and P450 11B2 by phospholipid
    Peng, Hwei-Ming
    Barlow, Chase
    Auchus, Richard J.
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2018, 181 : 63 - 72
  • [9] Human Cytochromes P450: Focus on Cytochrome P450 2C19
    Zvyaga, Tatyana
    Chang, Shu-Ying
    Chen, Cliff
    Yang, Zheng
    Vuppugalla, Ragini
    Hurley, Jeremy
    Thorndike, Denise
    Wagner, Andrew
    Chimalakonda, Anjaneya
    Rodrigues, A. David
    DRUG METABOLISM AND DISPOSITION, 2012, 40 (09) : 1698 - 1711
  • [10] Cytochrome P450 in the brain:: 2B or not 2B
    Hedlund, E
    Gustafsson, JÅ
    Warner, M
    TRENDS IN PHARMACOLOGICAL SCIENCES, 1998, 19 (03) : 82 - 85
12345