Physical association of G(i2)alpha with interleukin-8 receptors

Interleukin-8 (IL-8), one of the major mediators of the inflammatory response, belongs to a family of chemokines that includes NAP-2 (neutrophil-activating peptide-2) and Gro-alpha and whose biological activities are directed to a great extent toward neutrophils. Two distinct receptors have been described with overlapping, but not identical, binding affinities for IL-8, NAP-2, and Gro-alpha. This study was designed to examine the intracellular pathways activated upon the occupation of each of the IL-8 receptors (IL-8R). The formation of a physical coupling between IL-8 receptors and the alpha-subunit of heterotrimeric G proteins was tested in neutrophils by examining the presence of the former in anti-G alpha immune precipitates. The addition of IL-8 to a suspension of human neutrophils led to a time-dependent detection of IL-8 in anti-G(i2)alpha (raised against amino acids 159-168 (LERIAQSDYI) of G(i2)alpha) and anti-G(t) alpha (raised against the COOH-terminal 10 amino acids (KENLKDCGLF) of G(t) alpha), but not anti-G(q), immunoprecipitates. Similar results were obtained in human 293 cells stably transfected with IL-8RA or IL-8RB. The peptide derived from the COOH-terminal sequence of G(t) inhibited the co-immunoprecipitation of IL-8R and G(i) observed in response to the anti-G(t) alpha and anti-G(i2)alpha antibodies. On the other hand, the G(i2)alpha peptide only inhibited the immunoprecipitation induced by the anti-G(i2)alpha antibody. Peptides derived from G(i1)alpha or G(i3)alpha had no effect in this assay. The introduction of the anti-G(i2)alpha or anti-(t) alpha antibodies or their neutralizing peptides, but not the G(i1)alpha or G(i3)alpha peptides, into 293 IL-8RA or 293 IL-8RB cells completely blocked the calcium responses obtained upon stimulation with IL-8. These results demonstrate that the occupation of either type of IL-8 receptor leads to a physical coupling to the alpha-subunit of G(i2). In addition, the use of the subunit-specific peptides identified two functionally important but distinct regions of G(i) alpha, one involved in receptor/G(i) alpha interaction (KENLKDCGLF) and the other mediating downstream signal transmission (LERIAQSDYI). Finally, the results of this study also validate the use of the transfected 293 cell line as a model for the study of the signal transduction pathway(s) initiated by IL-8.