Synergistic effect of BMP9 and TGF-β in the proliferation and differentiation of osteoblasts

被引：24
|
作者：
Li, X. L. [1 ]
Liu, Y. B. [2 ]
Ma, E. G. [3 ]
Shen, W. X. [4 ]
Li, H. [5 ]
Zhang, Y. N. [1 ]
机构：
[1] Harbin Med Univ, Affiliated Hosp 2, Cadre Ward, Harbin, Peoples R China
[2] Harbin Med Univ, Affiliated Hosp 2, Dept Cardiol, Harbin, Peoples R China
[3] Harbin First Hosp, Dept Urinary Surg, Harbin, Peoples R China
[4] Harbin Med Univ, Affiliated Hosp 2, Med Oncol Cardiol, Harbin, Peoples R China
[5] Profess Judicial Police Coll Heilongjiang, Hlth Clin, Harbin, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 03期
关键词：
Bone morphogenetic protein 9; Cell differentiation; Mesenchymal stem cells; Mitogen activated protein kinase; Signal transduction pathway; MESENCHYMAL STEM-CELLS; BMP9-INDUCED OSTEOGENIC DIFFERENTIATION;
D O I：
10.4238/2015.July.13.4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigated the synergistic effect of bone morphogenetic protein 9 (BMP9) and transforming growth factor (TGF)-beta in the transformation of mesenchymal stem cells into osteoblasts. We evaluated the effect of BMP9 and TGF-beta on the induction of osteoblast formation. Mitogen-activated protein kinase (MAPK) pathway-related proteins such as p38, extracellular receptor kinase 1/2, and c-Jun N-terminal kinase (JNK) were analyzed. The interactions between TGF-Smad and BMP-MAPK were also studied. BMP9 alone induced the differentiation of mesenchymal stem cells (MSCs) into osteoblasts and enhanced phosphorylation of p38, extracellular receptor kinase 1/2, and JNK. TGF-beta alone failed to induce transformation, but could increase the effect of BMP9. In this process the activation of Smad resulted in activation of the JNK pathway in the MAPK pathway. BMP9 induced osteogenesis of MSC differentiation through the MAKP pathway, while TGF-beta contributed to BMP9 enhancement through the Smad-JNK pathway.
引用
收藏
页码：7605 / 7615
页数：11
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