Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

被引：0
|
作者：
Yoshimura, Aya [1 ]
Tamai, Yoshitaka [2 ]
Ochiya, Takahiro [3 ]
机构：
[1] Fujita Hlth Univ, Educ & Res Ctr Anim Models Human Dis, Toyoake, Aichi, Japan
[2] NIKON CeLL Innovat Co Ltd, Tokyo, Japan
[3] Tokyo Med Univ, Dept Mol & Cellular Med, Tokyo, Japan
来源：
EXTRACELLULAR VESICLES | 2020年 / 645卷
关键词：
EXOSOMES;
D O I：
10.1016/bs.mie.2020.09.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Extracellular vesicles (EVs) are mediators of intercellular communication in diverse cellular functions. Visualizing EVs in vivo is important to elucidate the biogenesis of EVs, and various approaches have been developed for in vivo EV tracking. The ubiquitously expressed tetraspanin CD63 is liberally incorporated into EVs. Thus, fluorescently tagged CD63 has been used in many studies to label EVs. In the present study, we presented isolation and transfer assays for EVs from two transgenic rats expressing CD63-GFP in their body fluids or brains.
引用
收藏
页码：231 / 242
页数：12
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