A method for calculation of solvent accessibility of aromatic amino acid residues of proteins in water-organic mixtures

被引：0
|
作者：
Shevchenko, AA [1 ]
Kost, OA [1 ]
机构：
[1] MOSCOW MV LOMONOSOV STATE UNIV,SCH CHEM,MOSCOW 119899,RUSSIA
来源：
BIOCHEMISTRY-MOSCOW | 1996年 / 61卷 / 12期
关键词：
solvent accessibility; water-organic media; derivative spectroscopy; tryptophan; tyrosine;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for calculation of solvent accessibility of tryptophan and tyrosine residues of a protein in water-organic mixtures based on second derivative UV spectra is suggested. The second derivative spectra can be used to distinguish the direct effect of an organic solvent on spectral characteristics of exposed aromatic amino acid residues in proteins from its effect on protein conformation. The method can be used for calculation of the exposure of tryptophan residues in water-organic mixtures for proteins containing no more tyrosine residues than tryptophan residues. A similar method for calculation of the exposure of tyrosine residues can be used for proteins with tyrosine content at least fivefold exceeding that of tryptophan. As an example, the solvent accessibility of tryptophan residues in chymotrypsin is calculated in the presence of various organic solvents e.g. ethanol, dioxane, and dimethyl formamide.
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页码：1502 / 1507
页数：6
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