Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?

被引：27

作者：

Thierens, H. ^{[1
]}

Vral, A. ^{[1
]}

Vandevoorde, C. ^{[1
]}

Vandersickel, V. ^{[1
]}

de Gelder, V. ^{[1
]}

Romm, H. ^{[2
]}

Oestreicher, U. ^{[2
]}

Rothkamm, K. ^{[3
]}

Barnard, S. ^{[3
]}

Ainsbury, E. ^{[3
]}

Sommer, S. ^{[4
]}

Beinke, C. ^{[5
]}

Wojcik, A. ^{[6
]}

机构：

[1] Univ Ghent, Dept Basic Med Sci, B-9000 Ghent, Belgium

[2] Bundesamt Strahlenschutz, Salzgitter, Germany

[3] Publ Hlth England, London, England

[4] Inst Nucl Chem & Technol, PL-03195 Warsaw, Poland

[5] Bundeswehr Inst Radiobiol, Munich, Germany

[6] Stockholm Univ, S-10691 Stockholm, Sweden

来源：

RADIATION PROTECTION DOSIMETRY | 2014年 / 159卷 / 1-4期

关键词：

DICENTRIC CHROMOSOMES; IMAGE-ANALYSIS; BIODOSIMETRY; RADIATION; LYMPHOCYTES; IRRADIATION; DOSIMETRY; EXPOSURE; NETWORK; TOOL;

D O I：

10.1093/rpd/ncu130

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution sigma(2)/A mu obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.

引用

页码：87 / 94

页数：8

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