Determination of molecular weights of C hordeins by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)

被引：6

作者：

Savage, AWJ ^{[1
]}

Saletti, R

Foti, S

Shewry, PR

Tatham, AS

机构：

[1] Univ Bristol, IACR Long Ashton Res Stn, Dept Agr Sci, Bristol BS41 9AF, Avon, England

[2] Univ Catania, Dipartimento Sci Chim, I-95125 Catania, Italy

来源：

CEREAL CHEMISTRY | 2002年 / 79卷 / 06期

关键词：

D O I：

10.1094/CCHEM.2002.79.6.768

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The molecular weights of nine C hordein proteins, including all of the major components identified by SDS-PAGE have been determined by MALDI-MS. Most were in the range 43,561 to 46,857, with a single component of M-r 57,606. These are higher than the masses previously reported based on the sequences of cloned genes. This indicates that reliance on masses determined solely by gene sequencing is not valid, presumably because these may not encode the major expressed proteins.

引用

页码：768 / 770

页数：3

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