Study of correlations between fine structure of bonded layer and affinity properties of silicas with attached cyclic oligopeptides

被引：3
|
作者：
Mingalyov, PG [1 ]
Orishchenko, NV [1 ]
Fadeev, AY [1 ]
机构：
[1] RES INST HAEMATOL & BLOOD TRANSFUS,UA-290044 LVOV,UKRAINE
来源：
JOURNAL OF CHROMATOGRAPHY A | 1997年 / 777卷 / 02期
关键词：
stationary phases; LC; silica; affinity adsorbents; oligopeptides; peptides; trypsin; thrombin; subtilisin;
D O I：
10.1016/S0021-9673(97)00257-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The cyclic oligopeptides bacitracin A and gramicidin S were immobilized on epoxy-, tosyloxy- and halogenoalkyl-activated silicas. The sorbents obtained were tested for affinity binding of proteinases. The effects of spacer length, ligand loading and the character of ligand attachment (one-or poly-site) on the biospecific characteristics have been studied. (C) 1997 Elsevier Science B.V.
引用
收藏
页码：249 / 259
页数：11
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