CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

被引：77
|
作者：
Ting, AH
Jair, KW
Suzuki, H
Yen, RWC
Baylin, SB [1 ]
Schuebel, KE
机构：
[1] Johns Hopkins Univ, Program Cellular & Mol Med, Baltimore, MD 21231 USA
[2] Johns Hopkins Univ, Canc Biol Div, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA
来源：
NATURE GENETICS | 2004年 / 36卷 / 06期
关键词：
D O I：
10.1038/ng1365
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.
引用
收藏
页码：582 / 584
页数：3
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