Benzylcarbamothioethionate from root bark of Moringa oleifera Lam. and its toxicological evaluation

A new compound, benzylcarbamothioethionate (1), was isolated from the chloroform soluble fraction of the ethanolic extract of the root bark of Moringa oleifera Lam. Its structure was established on the basis of 1D and 2D-NMR and mass spectroscopy. The acute toxicity studies of chloroform extract and compound 1 were performed on Long Evan's rats using four groups (two controls and two experimental). The hematological parameters such as the total RBC (red blood cell), total WBC (white blood cell), differential count of WBC, platelet count, hemoglobin and ESR (erythrocytes sedimentation rate) remained unchanged in both experimental and control groups. In case of biochemical study, SGPT (serum glutamate pyruvate transaminase), SGOT (serum glutamateoxaloacetate transaminase), SALP (serum alkaline phosphatase), bilirubin, creatinine and blood urea of experimental groups were also similar to that of control groups. Histopathology of liver, kidney, heart and lung did not reveal acute toxicity. So this study revealed that both the chloroform soluble fraction and compound 1 of Moringa oleifera Lam. had no toxic effects in our experimental models.