Inhibitory effects of Ginkgo biloba extract on vascular endothelial growth factor in rat aortic endothelial cells

被引：0
|
作者：
Zhang, L [1 ]
Rui, YC [1 ]
Yang, PY [1 ]
Qiu, Y [1 ]
Li, TJ [1 ]
Liu, HC [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Pharmacol, Shanghai 200433, Peoples R China
来源：
ACTA PHARMACOLOGICA SINICA | 2002年 / 23卷 / 10期
关键词：
Ginkgo biloba; vascular endothelium; endothelial growth factors; lysophosphatidylcholines; enzyme-linked immunosorbent assay; in situ hybridization;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To study the protective effects of Ginko biloba extract (GbE) against rat aortic endothelial cells (RAEC) damage induced by lysophosphatidylcholine (LPC). METHODS: Cell injury were determined by MTT assay and LDH release. Vascular endothelial growth factor (VEGF) protein production from RAEC was determined by enzyme-Linked immunosorbent assay (ELISA). VEGF mRNA expression was examined by in situ hybridization and dot blot. RESULTS: GbE 0.01-1 mug/L prevented LPC-induced injury in cultured RAEC in a concentration-dependent manner. Cultured RAEC could express VEGF protein and VEGF mRNA was induced by LPC 5 mg/L. GbE could inhibit the expression of VEGF protein and VEGF mRNA in co-cultured RAEC with LPC. CONCLUSION: LPC could induce a strong expression of VEGF in RAEC. GbE could protect RAEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured RAEC.
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页码：919 / 923
页数：5
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