Acetobacter vaccinii sp. nov., a novel acetic acid bacterium isolated from blueberry fruit (Vaccinium corymbosum L.)

Strain C17-3T was isolated from blueberry fruits collected from a farmland located in Damyang- gun, Jeollanam- do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain C17- 3T to the genus Acetobacter, where it occupied a rather isolated line of descent with Acetobacter ghanensis 430AT and Acetobacter lambici LMG 27439T as the nearest neighbours (98.9 % sequence similarity to both species). The highest average nucleotide identity and digital DNA-DNA hybridi-zation values were 76.3 % and 21.7 % with Acetobacter garciniae TBRC 12339T; both values were well below the cutoff values for species delineation. Cells are strictly aerobic, Gram- stain-negative rods, catalase-positive and oxidase-negative. The DNA G+C content calculated from the genome sequence was 59.2 %. Major fatty acids were summed feature 8 (C18:1 & omega;6c and/or C18:1 & omega;7c) and C19:0cyclo & omega;8c. The major isoprenoid quinone was ubiquinone 9. On the basis of the results of phylogenetic analyses, pheno-typic features and genomic comparisons, it is proposed that strain C17- 3T represents a novel species of the genus Acetobacter and the name Acetobacter vaccinii sp. nov. is proposed. The type strain is C17- 3T (= KACC 21233T = LMG 31758T).