Identification of novel HrpXo regulons preceded by two cis-acting elements, a plant-inducible promoter box and a-10 box-like sequence, from the genome database of Xanthomonas oryzae pv. oryzae

被引：45

作者：

Furutani, A

Nakayama, T

Ochiai, H

Kaku, H

Kubo, Y

Tsuge, S ^{[1
]}

机构：

[1] Kyoto Prefectural Univ, Grad Sch Agr, Plant Pathol Lab, Kyoto 6068522, Japan

[2] Ibaraki Univ, Coll Agr, Ami, Ibaraki 30003, Japan

[3] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 259卷 / 01期

关键词：

Xanthomonas oryzae pv. oryzae; type III secretion system; plant-inducible promoter box; HrpXo regulons;

D O I：

10.1111/j.1574-6968.2006.00265.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae, searching for ORFs preceded by two predicted sequence motifs, a plant-inducible promoter box-like sequence and a -10 box-like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base-substituted mutagenesis that both motifs are essential for the expression of the genes.

引用

页码：133 / 141

页数：9