To assess the reliability and validity of the Tree-Drawing Test in medical college students. Methods The study randomly selected 312 aged 19 to 23-year-old medical students to take part in Tree-Drawing Test. In addition, a total of 275 college students were selected to receive re-test, the Pearson correlation coefficient of two tests were calculated after 30 days. Three raters were invited to assess 30 trees painting score, analyzing the Kendall coefficient of concordance between the scores to verify raters' reliability; parts of students also participated in the 16PF test, SAS, SDS test, analyzing the correlation coefficient between the various test results, in order to assess the effectiveness of the Tree-Drawing Test. Conclusion: The re-test reliability is between 0.570 and 0.733, the raters' reliability is between 0.491 and 0.626, there are some correlations between Tree-Drawing Test and 16PF, SAS, SDS. Tree-Drawing Test in extroverted personality test, including anxiety, depression, aggressive has good reliability and validity, reached psychometric standards. It can be applied to the detection of college students' psychological assessment and psychological problems.