Potential Pathogen Bacteria During the Dry Period in Dual Purpose Cows With Subclinical Mastitis

Scientific research in milk production has been evolving over more than 100 years to attain the highest amount of milk/cow/year along with an excellent health status of the mammary gland. This health status of the mammary gland is affected by parenchymal inflammation, i.e. mastitis, caused in general by bacteria, such as Staphylococcus spp., Streptococcus spp., Corynebacterium spp. and enterobacteria. The objective of this trial was to determine the bacterias present in mammary glands with subclinical mastitis at the beginning of the dry period. Thirty nine (39) multiparous crossbred dual purpose cows at the end of the lactating period were randomly selected; they had a seven-month gestation, and they were negative at the black bottom cup test; they have a >= +2 score at the CMT as well. Milk samples were taken for bacteriological study. Bacterial growth was observed in milk sample from 96 udder quarters (61.53%) and in milk sample from 49 quarters there was no growth (31.41%). The most frequently isolated bacteria were Corynebacterium bovis (46.73%), Staphylococcus epidermidis (20.56%), Staphylococcus aureus (12.15%) and Arcano-bacterium pyogenes (7.48%). As for growth type, 88.54% of cultures were pure cultures and 11.46% were mixed cultures. As for pathogenicity potential, 73.82% of the isolates were minor pathogens and 18-69% were major pathogens. In conclusion, bacteria in mammary glands with subclinical mastitis at the dry period, their distribution as for genus and species, growth type and pathogenicity potential were identified. On other hand, these bacteria can generate clinical mastitis in the lactating period with the resulting decrease in the quality and quantity of yielded milk.