Production of cyclodextrin from starch by cyclodextrin glycosyltransferase from Bacillus firmus and characterization of purified enzyme

被引：37
|
作者：
Yim, DG
Sato, HH
Park, YH
Park, YK
机构：
[1] STATE UNIV CAMPINAS,COLL FOOD ENGN,UNICAMP,BR-13081970 CAMPINAS,SP,BRAZIL
[2] KOREA RES INST BIOSCI & BIOTECHNOL,TAEJON,SOUTH KOREA
来源：
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1997年 / 18卷 / 06期
关键词：
cyclodextrin; cyclodextrin glycosyltransferase; starch; alkalophilic bacillus; Bacillus firmus;
D O I：
10.1038/sj.jim.2900400
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
During screening for cyclodextrin-forming microorganisms, an alkalophilic Bacillus sp, which produced high activity of cyclodextrin glycosyltransferase, was isolated and identified as Bacillus firmus. The crude enzyme transformed starch to mainly beta-and gamma-cyclodextrin. The purified enzyme had an optimum pH of 7.5-8.5 and its optimum temperature was 65 degrees C, which is the highest optimum temperature as compared to other cyclodextrin glycosyltransferases except that produced by Bacillus amyloliquefaciens.
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页码：402 / 405
页数：4
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