In vitro Ovicidal and Wormicidal Activity of Six Medicinal Plants against Haemonchus contortus

The present research was conducted to evaluate the anthelmintic activity of six Pakistani plant extracts viz. Azadirachta indica, Adhatoda vasica, Nicotiana tabacum, Saussuria lappa, Terminalia chebula and Convolvulus arvensis. Dried plant material was ground finely in powder and aqueous-methanol extracts were prepared by cold maceration method. In vitro anthelmintic activity was evaluated against Haemonchus contortus of sheep using adult motility assay (AMA) and egg hatch test (EHT). Varying degree of anthelmintic activity was observed for all the selected plants. In EHT, based on the LC50 values, most effective plants (LC50 in ppm) in their order of activity were; N. tabacum (0.10), S. lappa (0.73), A. indica (1.73), C. arvensis (2.51), T. chebula (5.55) and A. vasica (15.74). LC50 values of three plants viz. N. tabacum, S. lappa and A. indica were statistically similar to LC50 of oxfendazole (0.38). In AMA, a time dependent response was observed in all the treatment groups. (C) 2014 Friends Science Publishers