Streptomyces venezuelae ISP5230 Maintains Excretion of Jadomycin upon Disruption of the MFS Transporter JadL Located within the Natural Product Biosynthetic Gene Cluster

被引：8

作者：

Forget, Stephanie M. ^{[1
]}

McVey, Jennifer ^{[2
,4
]}

Vining, Leo C. ^{[2
]}

Jakeman, David L. ^{[1
,3
]}

机构：

[1] Dalhousie Univ, Dept Chem, Halifax, NS, Canada

[2] Dalhousie Univ, Dept Biol, Halifax, NS, Canada

[3] Dalhousie Univ, Coll Pharm, Halifax, NS, Canada

[4] Dalhousie Univ, Halifax Infirm, Dept Emergency Med, Halifax, NS, Canada

来源：

FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

natural products; MFS transporters; streptomyces; drug efflux; jadomycins; major facilitator superfamily; DISCOVERY; ACTIVATION; INSIGHTS; CLONING; FAMILY;

D O I：

10.3389/fmicb.2017.00432

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

JadL was identified as a Major Facilitator Superfamily (MFS) transporter (T.C. 2.A.1) through sequence homology. The protein is encoded by jadL, situated within the jadomycin biosynthetic gene cluster. JadL has, therefore, been assigned a putative role in host defense by exporting its probable substrates, the jadomycins, a family of secondary metabolites produced by Streptomyces venezuelae ISP5230. Herein, we evaluate this assumption through the construction and analysis of a jadL disrupted mutant, S. venezuelae VS678 (Delta jadL::aac(3)IV). Quantitative determination of jadomycin production with the jadL disrupted mutant did not show a significant decrease in production in comparison to the wildtype strain, as determined by HPLC and by tandem mass spectrometry. These results suggest that efflux of jadomycin occurs upon disruption of jadL, or that JadL is not involved in jadomycin efflux. Potentially, other transporters within S. venezuelae ISP5230 may adopt this role upon inactivation of JadL to export jadomycins.

引用

页数：8