An experimental assessment of suture techniques using Lapra-ty® clips through the laparoscope and a case report:: Laparoscopic closure using Lapra-ty® for a perforation of the sigmoid colon

Background/Aims:, As endoscopic procedures become various and complicated, it becomes necessary to develop alternative techniques for tissue suture and ligation. We have developed a new suture technique intracorporeal knotting. Methodology: An 8-cm suture material having a knot on the other side of a nee 16, was used in laparoscopic surgery. Furthermore, a 5-mm(2) Vicryl(R) mesh was pierced as a buttress. The Lapra-ty(R) was used to secure the tissue. Under laparotomy, the forestomach of a male Donryu rat at 6 weeks of age was incised and sutured with 3-0 Vicryl(R). 3-0 Vicryl(R) was secured with the knot substitute (group, A) or three-throw knot (group B) under Nembutal anesthesia. The animals were sacrificed 7 days after operation. Specimens were taken from the forestomach. Those were fixed with 10% formalin and stained with hematoxylin eosin and Elastica Wangieson methods. The histological findings were compared between group A and group B. A successful laparoscopic Closure using Lapra-ty(R) is reported in a patient with a perforation of the sigmoid colon. Results: Microscopically, in this method, neither erosion, ulcer nor microabscess is evidenced. This method may make an intracorporeal knot easy. Also we treated a patient. who had a perforation of the sigmoid colon using Lapra-ty(R). The patient recovered without any trouble. Conclusions: This, method could make ligation technique much easier and the operating time shorter.