Carbon nanotube-based DNA biosensor for monitoring phenolic pollutants

被引：29
|
作者：
Zheng, Yanqiong [1 ]
Yang, Changzhu [1 ]
Pu, Wenhong [1 ]
Zhang, Jingdong [2 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Environm Sci & Engn, Wuhan 430074, Peoples R China
[2] Huazhong Univ Sci & Technol, Coll Chem & Chem Engn, Wuhan 430074, Peoples R China
来源：
MICROCHIMICA ACTA | 2009年 / 166卷 / 1-2期
关键词：
Direct electrochemistry; DNA biosensor; MWNTs modified electrode; Determination of phenols; SINGLE-STRANDED-DNA; DIRECT ELECTROCHEMISTRY; PASTE; ELECTRODE; WATER; TOXICANTS; SENSOR;
D O I：
10.1007/s00604-009-0154-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A DNA biosensor was constructed by immobilizing DNA on a glassy carbon (GC) electrode modified with multiwall carbon nanotubes (MWNTs) dispersed in Nafion (DNA/MWNTs/GCE). The DNA-modified electrode exhibited two well-defined oxidation peaks corresponding to the guanine and adenine residues of DNA, respectively. The effects of the adsorption potential, DNA concentration and quantity of MWNTs used for DNA immobilization were investigated, as were the effects of buffer, pH and scan rate on the voltammetric behavior of DNA. Phenol, m-cresol and catechol showed noticeable inhibition towards the response of the electrode due to their interactions with DNA. These findings were used to design biosensors with linear response to these phenolic pollutants.
引用
收藏
页码：21 / 26
页数：6
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