The importance of careful blood processing in isolation of cell-free DNA

被引：53
|
作者：
Page, Karen
Powles, Tom
Slade, Martin J.
De Bella, Manuela Tamburo
Walker, Rosemary A.
Coombes, R. Charles
Shaw, Jacqueline A.
机构：
[1] Univ Leicester, Dept Canc Studies & Mol Med, Leicester LE2 7LX, Leics, England
[2] Imperial Coll Sch Med, Dept Canc Med, Sch Med, London W12 0NN, England
来源：
CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV | 2006年 / 1075卷
关键词：
cell-free plasma DNA; PCR; apoptosis;
D O I：
10.1196/annals.1368.042
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In healthy individuals, the source of cell-free plasma DNA is predominantly apoptotic, whereas, increased plasma DNA integrity is seen in cancer patients. Therefore, it is important to carefully isolate absolutely "cell-free" plasma DNA. Plasma DNA from 30 healthy females was analyzed using 4 PCR amplicons of increasing size, comparing standard blood processing with additional centrifugation steps prior to DNA extraction. Cellular DNA contamination, indicated by positive amplicons > 300 bp was eliminated only after the extra centrifugation step. This highlights the importance of careful processing in preparation of cell-free plasma DNA as a tool for cancer detection and we recommend the use of a microcentrifuge spin, prior to DNA extraction.
引用
收藏
页码：313 / 317
页数：5
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