Autoinducer-2 bioassay is a qualitative, not quantitative method influenced by glucose

被引:50
|
作者
Turovskiy, Yevgeniy [1 ]
Chikindas, Michael L. [1 ]
机构
[1] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA
关键词
nisin; Listeria monocytogenes; stress response; AI-2; quorum sensing; autoinducer-2; ATR; acid tolerance response;
D O I
10.1016/j.mimet.2006.02.001
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Autoinducer-2 (AI-2) is a cell-to-cell signaling molecule which is thought to be utilized for quorum sensing processes by a variety of prokaryotic species. This molecule is usually detected using a so-called autoinducer bioassay, which relies on the ability of a Vibrio harveyi reporter strain to produce light in response to AI-2. However, as previously reported, the presence of glucose in the sample can inhibit the bioluminescence of the reporter strain, a fact that is often ignored by investigators. Our data suggest that the presence of glucose in concentrations below that required for the inhibition of bioluminescence may lead to incorrect AI-2 readings and produce misleading (false-positive) results. Our findings also suggest that even if all the limitations of this bioassay are considered, the large standard deviation of the method allows only for a qualitative and not quantitative interpretation of the obtained results. (c) 2006 Elsevier B.V All rights reserved.
引用
收藏
页码:497 / 503
页数:7
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