Assessment of heterosis proteins in maize (Zea mays L.) leaves by two-dimensional gel electrophoresis

被引:1
|
作者
Bandehagh, Ali [1 ]
Dehghanian, Zahra [2 ]
Moharramnejad, Sajjad [3 ]
Rad, Shiva Aliyari [2 ]
Shirmohammadi, Shahram [4 ]
Ashraf, Muhammad [5 ]
机构
[1] Univ Tabriz, Fac Agr, Dept Plant Breeding & Biotechnol, Tabriz, Iran
[2] Azarbaijan Shahid Madani Univ, Fac Agr, Dept Biotechnol, Tabriz, Iran
[3] AREEO, Ardabil Agr & Nat Resources Res & Educ Ctr, Crop & Hort Sci Res Dept, Moghan, Iran
[4] Univ Tabriz, Fac Agr, Tabriz, Iran
[5] Univ Agr Faisalabad, Faisalabad, Pakistan
关键词
Defense system; Hybrid; Inbred line; Metabolism; Proteomics; HYBRID; STARCH;
D O I
10.1016/j.plgene.2021.100331
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In the current research, the pattern of expression of hybrid proteins in the leaves of two maize inbred lines, B73 (female) and MO17 (male), as well as the F1, SC704, was investigated using 2-DE. A hydroponic experiment was conducted in a greenhouse. Leaf tissue proteome analysis of the two parents and their F1 showed that seven reproducible protein spots had significant modifications, revealing their up-regulation. These seven differentially expressed proteins were categorized into three functional groups, including the defense system, regulator, and energy metabolism. Proteomic analysis indicated that all proteins of the mid parent had prominent expression compared with that of the parental proteins. It can be concluded that in the significant vigor of hybrid SC704 was due to enhanced expression of proteins involved in defense, regulation, and energy metabolism.
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页数:8
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