Identification and characterization of cis-regulatory elements insulator and repressor' in PPARD gene

被引：5

作者：

Yadav, Dilip K. ^{[1
]}

Shrestha, Smeeta ^{[1
,2
]}

Dadhwal, Gunjan ^{[1
,3
]}

Chandak, Giriraj R. ^{[1
]}

机构：

[1] Ctr Cellular & Mol Biol, CSIR, Genom Res Complex Dis GRC Grp, Hyderabad 500007, Telangana, India

[2] Dayananda Sagar Univ, Sch Basic & Appl Sci, Bldg 7,Shavige Malleshwara Hills, Bangalore 560078, Karnataka, India

[3] Univ Montreal, Dept Biochim & Med Mol, Montreal, PQ H3T 1J4, Canada

来源：

EPIGENOMICS | 2018年 / 10卷 / 05期

关键词：

cis-regulatory elements; ENCODE; enhancer; insulator; peroxisome proliferator-activated receptor beta/delta; repressor; Type; 2; diabetes; PROLIFERATOR-ACTIVATED RECEPTORS; ENHANCER-BLOCKING ACTIVITY; TRANSCRIPTIONAL REGULATION; GENOME BROWSER; DELTA; CTCF; METHYLATION; METABOLISM; BOUNDARIES; PROTEIN;

D O I：

10.2217/epi-2017-0139

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: Identification and functional characterization of cis-regulatory elements in human PPARD gene. Methods: We used various bioinformatic tools on the publicly available human genome and Encyclopedia of DNA Elements databases to explore potential cis-regulatory elements in PPARD gene region. Results: We predicted an insulator and an enhancer element in intron 2 of PPARD gene. Functional characterization using transient transfection, reporter assay and CTCF binding confirmed the insulator status. However, the predicted enhancer element showed repressor/silencer activity. Finally, we observed a potential interaction between these two cis-regulatory elements which is in agreement with 5C-Encyclopedia of DNA Elements data. Conclusion: We report two functionally validated cis-regulatory elements in PPARD gene which will aid in understanding its regulation and role in metabolic functions.

引用

页码：613 / 627

页数：15

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