Comparison of the solution structures of a DNA dodecamer using NOE and residual dipolar coupling data

被引:3
|
作者
Alvarez-Salgado, F [1 ]
Berthault, P
Boulard, Y
Desvaux, H
机构
[1] CEA Saclay, Serv Chim Mol, DRECAM, DSM,Lab Commun RMN, F-91191 Gif Sur Yvette, France
[2] CEA Saclay, Serv Biochim & Genet Mol, DBJC, DSV,Lab Commun RMN, F-91191 Gif Sur Yvette, France
关键词
NMR; DNA; dilute liquid crystals; residual dipolar couplings;
D O I
10.1016/j.crci.2003.12.007
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The solution structure of a DNA dodecamer is determined by combining dipolar cross-relaxation and residual dipolar coupling (RDCs) data. The C-13-H-1 RDCs result from the partial alignment of the molecule after its dissolution in different oriented dilute liquid crystals: polyethylene glycols, bicelles and phages. It clearly appears that thanks to the use of RDCs, the global curvature of the duplex solution structures is reduced. This is shown to result from the use of long-range RDC restraints and from the symmetry properties of a self-complementary dodecamer. (C) 2004 Academie des sciences. Published by Elsevier SAS. All rights reserved.
引用
收藏
页码:259 / 263
页数:5
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