A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

被引：40
|
作者：
Dai, Xi [1 ]
Zhang, Tao [2 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
机构：
[1] Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China
[2] Shandong Univ, Inst Dev Biol, Sch Life Sci, Jinan 250100, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 223卷
基金：
中国国家自然科学基金;
关键词：
Ratiometric fluorescence; Two photon; Aqueous solution; Cell imaging; 2-PHOTON ABSORPTION; LIVING CELLS; THIOL PROBE; CYSTEINE; GLUTATHIONE; CHEMODOSIMETER; PHTHALOCYANINE; HOMOCYSTEINE; DERIVATIVES; THIOPHENOLS;
D O I：
10.1016/j.snb.2015.09.106
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10(-8)M and two-photon 10(-7)M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：274 / 279
页数：6
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