A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

被引：11

作者：

Sun, Ning ^{[1
]}

Guo, Qi ^{[2
]}

Shao, Jingwei ^{[3
]}

Qiu, Bin ^{[1
,3
]}

Lin, Zhenyu ^{[3
]}

Wong, K. Y. ^{[1
]}

Chen, Guonan ^{[3
]}

机构：

[1] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, Dept Pharm, Guangzhou 510060, Guangdong, Peoples R China

[3] Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China

来源：

ANALYTICAL METHODS | 2014年 / 6卷 / 10期

基金：

国家科技攻关计划;

关键词：

GOLD NANOPARTICLES; ELECTROCHEMICAL SENSOR; ATP; PROBE; DNA; COPPER(II); STRATEGY; IONS;

D O I：

10.1039/c4ay00220b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(II). In the presence of sodium ascorbate, Cu(II) can be reduced to Cu(I) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results.

引用

页码：3370 / 3374

页数：5

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