Copper tolerance mediated by polyphosphate degradation and low-affinity inorganic phosphate transport system in Escherichia coli

被引:33
|
作者
Grillo-Puertas, Mariana [1 ,2 ]
Ariane Schurig-Briccio, Lici [1 ,2 ]
Rodriguez-Montelongo, Luisa [1 ,2 ]
Regina Rintoul, Maria [1 ,2 ]
Andrea Rapisarda, Viviana [1 ,2 ]
机构
[1] INSIBIO CONICET UNT, Inst Super Invest Biol, San Miguel De Tucuman, Argentina
[2] Fac Bioquim Quim & Farm UNT, Inst Quim Biol Dr Bernabe Bloj, San Miguel De Tucuman, Argentina
来源
BMC MICROBIOLOGY | 2014年 / 14卷
关键词
Escherichia coli; Copper tolerance; Stationary phase; Polyphosphate; Inorganic phosphate; STATIONARY-PHASE; GENE; EXPRESSION; RESISTANCE; METALS; HOMEOSTASIS; ENVIRONMENT; PROTEIN; EFFLUX; CELLS;
D O I
10.1186/1471-2180-14-72
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Metal tolerance in bacteria has been related to polyP in a model in which heavy metals stimulate the polymer hydrolysis, forming metal-phosphate complexes that are exported. As previously described in our laboratory, Escherichia coli cells grown in media containing a phosphate concentration > 37 mM maintained an unusually high polyphosphate (polyP) level in stationary phase. The aim of the present work was to evaluate the influence of polyP levels as the involvement of low-affinity inorganic phosphate transport (Pit) system in E. coli copper tolerance. Results: PolyP levels were modulated by the media phosphate concentration and/or using mutants in polyP metabolism. Stationary phase wild-type cells grown in high phosphate medium were significantly more tolerant to copper than those grown in sufficient phosphate medium. Copper addition to tolerant cells induced polyP degradation by PPX (an exopolyphosphatase), phosphate efflux and membrane polarization. ppk-ppx-(unable to synthesize/degrade polyP), ppx-(unable to degrade polyP) and Pit system mutants were highly sensitive to metal even in high phosphate media. In exponential phase, CopA and polyP-Pit system would act simultaneously to detoxify the metal or one could be sufficient to safeguard the absence of the other. Conclusions: Our results support a mechanism for copper detoxification in exponential and stationary phases of E. coli, involving Pit system and degradation of polyP. Data reflect the importance of the environmental phosphate concentration in the regulation of the microbial physiological state.
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页数:10
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