Microstructured arrays with pre-synthesized capture probes for DNA detection based on metal nanoparticles and silver enhancement

被引：14
|
作者：
Zhang, GJ [1 ]
Möller, R [1 ]
Kretschmer, R [1 ]
Csáki, A [1 ]
Fritzsche, W [1 ]
机构：
[1] Inst Phys High Technol, D-07702 Jena, Germany
来源：
JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 04期
关键词：
DNA microarray; gold nanoparticles; micro structures;
D O I：
10.1023/B:JOFL.0000031818.39925.4c
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.
引用
收藏
页码：369 / 375
页数：7
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