Cloning and Characterization of the Promoter of the 9-cis-Epoxycarotenoid Dioxygenase Gene in Arachis hypogaea L.

被引:19
|
作者
Liang, Jianhua [1 ]
Yang, Lixia [1 ]
Chen, Xiong [1 ]
Li, Ling [1 ]
Guo, Dongliang [1 ]
Li, Haihang [1 ]
Zhang, Biyu [1 ]
机构
[1] S China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Guangdong, Peoples R China
关键词
9-cis-epoxycarotenoid dioxygenase; promoter; water stress; abscisic acid response element; Arachis hypogaea L; ABSCISIC-ACID BIOSYNTHESIS; EXPRESSION; INDUCTION; SEQUENCE; ELEMENTS; DATABASE; DROUGHT;
D O I
10.1271/bbb.90133
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. beta-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by application of abscisic acid (ABA) and salicylic acid (SA) in transgenic Arabidopsis. Moreover, two novel ABRE-like (abscisic acid response element) elements were identified in the promoter region.
引用
收藏
页码:2103 / 2106
页数:4
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