Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen

被引：8
|
作者：
Consalter, Angelica [1 ]
Silva, Andressa F. [2 ]
Frazao-Teixeira, Edwards [3 ]
Matos, Luis F. [4 ]
de Oliveira, Francisco C. R. [5 ]
Leite, Juliana S. [6 ]
Silva, Franciele B. F. [7 ]
Ferreira, Ana M. R. [1 ,6 ]
机构：
[1] Univ Fed Fluminense, Programa Posgrad Med Vet Clin & Reprod Anim, Niteroi, RJ, Brazil
[2] Univ Fed Rural Rio Janeiro, Dept Med & Cirurgia Vet, Seropedica, RJ, Brazil
[3] IOC Fiocruz, Inst Oswaldo Cruz, Lab Biol Estrutural, Rio De Janeiro, RJ, Brazil
[4] Univ Estadual Norte Fluminense, Lab Reprod & Melhoramento Genet Anim, Campos Dos Goytacazes, Brazil
[5] Univ Estadual Norte Fluminense, Lab Sanidade Anim, Campos Dos Goytacazes, Brazil
[6] Univ Fed Fluminense, Lab Anat Patol Vet, Niteroi, RJ, Brazil
[7] Univ Fed Fluminense, Programa Posgrad Patol, Niteroi, RJ, Brazil
来源：
THERIOGENOLOGY | 2017年 / 90卷
关键词：
Toxoplasmosis; Ovine; Reproductive disease; Semen; IHC; PCR; RAMS; DNA; IDENTIFICATION; TISSUES; LESIONS;
D O I：
10.1016/j.theriogenology.2016.12.004
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evaluate if ram's frozen semen spiked with T. gondii tachyzoites would be able to cause infection in sheep by laparoscopic artificial insemination (AI). Nine ewes tested seronegative to anti-T. gondii antibodies by the modified agglutination test (MAT) were superovulated and inseminated to collect embryos. Animals were divided into two groups: G1 (n = 5), ewes inseminated with semen containing 4 x 10(7) tachyzoites; and G2 (n = 4), ewes inseminated with tachyzoite-free semen (control group). To confirm infection, ewe's blood samples were collected on days 14, 7, 0, 7,14, 21, 28, 35, 49 and 57 after AI for analysis by MAT and PCR. Tissue samples of these ewes were also collected for histopathology and immunohistochemistry (IHC). Seven days after AI, all ewes of group G1 had specific antibodies to T. gondii, while those of G2 were negative. Toxoplasma gondii DNA was detected in the blood of one ewe and parasites were observed in tissues of all five animals inseminated with contaminated semen, indicating that semen freezing protocol does not affect T. gondii transmission by artificial insemination in sheep. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：169 / 174
页数：6
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